LOCUS       40924_22889       11711 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Gateway binary vector pGWB412 DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11711)
  AUTHORS   Nakagawa T, Suzuki T, Murata S, Nakamura S, Hino T, Maeo K, Tabata 
            R, Kawai T, Tanaka K, Niwa Y, Watanabe Y, Nakamura K, Kimura T, 
            Ishiguro S.
  TITLE     Improved Gateway Binary Vectors: High-Performance Vectors for 
            Creation of Fusion Constructs in Transgenic Analysis of Plants
  JOURNAL   Biosci. Biotechnol. Biochem. (2007) In press
  PUBMED    17690442
REFERENCE   2  (bases 1 to 11711)
  AUTHORS   Nakagawa T, Ishiguro S.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2007) Tsuyoshi Nakagawa, Shimane University, 
            Center for Integrated Research in Science; 1060 Nishikawatsu, 
            Matsue, Shimane 690-8504, Japan 
            (E-mail:tnakagaw@life.shimane-u.ac.jp, Tel:81-852-32-6595, 
            Fax:81-852-32-6109)
REFERENCE   3  (bases 1 to 11711)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 11711)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biosci. 
            Biotechnol. Biochem. (2007) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (21-FEB-2007) Tsuyoshi Nakagawa, Shimane University, Center for 
            Integrated Research in Science"; volume: " 1060 Nishikawatsu, 
            Matsue, Shimane 690-8504, Japan 
            (E-mail:tnakagaw@life.shimane-u.ac.jp, Tel:81-852-32-6595, Fax"; 
            pages: "81-852-32-6109"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     constructed using pPZP221.
FEATURES             Location/Qualifiers
     source          1..11711
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    complement(54..78)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        812..1157
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             1178..1201
                     /codon_start=1
                     /label=FLAG
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
                     /translation="DYKDDDDK"
     protein_bind    1211..1335
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        1372..1402
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             1456..2112
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     CDS             2457..2759
                     /codon_start=1
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     protein_bind    complement(2803..2927)
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      2953..3205
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(3239..3255)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3263..3279
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3287..3317)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3332..3353)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     terminator      complement(3387..3639)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(4032..4820)
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase from Tn5"
                     /translation="IEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPV
                     LFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSS
                     HLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQG
                     LAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIAL
                     ATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     promoter        complement(4844..5027)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    complement(5233..5257)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             5778..6566
                     /codon_start=1
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      6815..7403
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(7589..7729)
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(8073..8267)
                     /direction=LEFT
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(8336..9406)
                     /codon_start=1
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
                     /translation="VSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(9838..10464)
                     /codon_start=1
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttgcat gcctgcaggt ccccagatta gccttttcaa tttcagaaag aatgctaacc
      361 cacagatggt tagagaggct tacgcagcag gtctcatcaa gacgatctac ccgagcaata
      421 atctccagga aatcaaatac cttcccaaga aggttaaaga tgcagtcaaa agattcagga
      481 ctaactgcat caagaacaca gagaaagata tatttctcaa gatcagaagt actattccag
      541 tatggacgat tcaaggcttg cttcacaaac caaggcaagt aatagagatt ggagtctcta
      601 aaaaggtagt tcccactgaa tcaaaggcca tggagtcaaa gattcaaata gaggacctaa
      661 cagaactcgc cgtaaagact ggcgaacagt tcatacagag tctcttacga ctcaatgaca
      721 agaagaaaat cttcgtcaac atggtggagc acgacacact tgtctactcc aaaaatatca
      781 aagatacagt ctcagaagac caaagggcaa ttgagacttt tcaacaaagg gtaatatccg
      841 gaaacctcct cggattccat tgcccagcta tctgtcactt tattgtgaag atagtggaaa
      901 aggaaggtgg ctcctacaaa tgccatcatt gcgataaagg aaaggccatc gttgaagatg
      961 cctctgccga cagtggtccc aaagatggac ccccacccac gaggagcatc gtggaaaaag
     1021 aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa
     1081 gggatgacgc acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat
     1141 ttcatttgga gagaacacgg gggactctag aatgagcgac tacaaggatg acgatgacaa
     1201 ggctgttatc acaagtttgt acaaaaaagc tgaacgagaa acgtaaaatg atataaatat
     1261 caatatatta aattagattt tgcataaaaa acagactaca taatactgta aaacacaaca
     1321 tatccagtca ctatggcggc ccctcgaggg gccgcattag gcaccccagg ctttacactt
     1381 tatgcttccg gctcgtataa tgtgtggatt ttgagttagg atccggcgag attttcagga
     1441 gctaaggaag ctaaaatgga gaaaaaaatc actggatata ccaccgttga tatatcccaa
     1501 tggcatcgta aagaacattt tgaggcattt cagtcagttg ctcaatgtac ctataaccag
     1561 accgttcagc tggatattac ggccttttta aagaccgtaa agaaaaataa gcacaagttt
     1621 tatccggcct ttattcacat tcttgcccgc ctgatgaatg ctcatccgga attccgtatg
     1681 gcaatgaaag acggtgagct ggtgatatgg gatagtgttc acccttgtta caccgttttc
     1741 catgagcaaa ctgaaacgtt ttcatcgctc tggagtgaat accacgacga tttccggcag
     1801 tttctacaca tatattcgca agatgtggcg tgttacggtg aaaacctggc ctatttccct
     1861 aaagggttta ttgagaatat gtttttcgtc tcagccaatc cctgggtgag tttcaccagt
     1921 tttgatttaa acgtggccaa tatggacaac ttcttcgccc ccgttttcac catgggcaaa
     1981 tattatacgc aaggcgacaa ggtgctgatg ccgctggcga ttcaggttca tcatgccgtc
     2041 tgtgatggct tccatgtcgg cagaatgctt aatgaattac aacagtactg cgatgagtgg
     2101 cagggcgggg cgtaaacgcg tggatccggc ttactaaaag ccagataaca gtatgcgtat
     2161 ttgcgcgctg atttttgcgg tataagaata tatactgata tgtatacccg aagtatgtca
     2221 aaaagaggtg tgctatgaag cagcgtatta cagtgacagt tgacagcgac agctatcagt
     2281 tgctcaaggc atatatgatg tcaatatctc cggtctggta agcacaacca tgcagaatga
     2341 agcccgtcgt ctgcgtgccg aacgctggaa agcggaaaat caggaaggga tggctgaggt
     2401 cgcccggttt attgaaatga acggctcttt tgctgacgag aacagggact ggtgaaatgc
     2461 agtttaaggt ttacacctat aaaagagaga gccgttatcg tctgtttgtg gatgtacaga
     2521 gtgatattat tgacacgccc gggcgacgga tggtgatccc cctggccagt gcacgtctgc
     2581 tgtcagataa agtctcccgt gaactttacc cggtggtgca tatcggggat gaaagctggc
     2641 gcatgatgac caccgatatg gccagtgtgc cggtctccgt tatcggggaa gaagtggctg
     2701 atctcagcca ccgcgaaaat gacatcaaaa acgccattaa cctgatgttc tggggaatat
     2761 aaatgtcagg ctcccttata cacagccagt ctgcaggtcg accatagtga ctggatatgt
     2821 tgtgttttac agtattatgt agtctgtttt ttatgcaaaa tctaatttaa tatattgata
     2881 tttatatcat tttacgtttc tcgttcagct ttcttgtaca aagtggtgat aacctagagc
     2941 tcgaatttcc ccgatcgttc aaacatttgg caataaagtt tcttaagatt gaatcctgtt
     3001 gccggtcttg cgatgattat catataattt ctgttgaatt acgttaagca tgtaataatt
     3061 aacatgtaat gcatgacgtt atttatgaga tgggttttta tgattagagt cccgcaatta
     3121 tacatttaat acgcgataga aaacaaaata tagcgcgcaa actaggataa attatcgcgc
     3181 gcggtgtcat ctatgttact agatcgggaa ttggttccgg aaccaattcg taatcatggt
     3241 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
     3301 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaggctgaa
     3361 ttaggcgcgc ctatttctga attcccgatc tagtaacata gatgacaccg cgcgcgataa
     3421 tttatcctag tttgcgcgct atattttgtt ttctatcgcg tattaaatgt ataattgcgg
     3481 gactctaatc ataaaaaccc atctcataaa taacgtcatg cattacatgt taattattac
     3541 atgcttaacg taattcaaca gaaattatat gataatcatc gcaagaccgg caacaggatt
     3601 caatcttaag aaactttatt gccaaatgtt tgaacgatcg gggatcatcc gggtctgtgg
     3661 cgggaactcc acgaaaatat ccgaacgcag caagatatcg cggtgcatct cggtcttgcc
     3721 tgggcagtcg ccgccgacgc cgttgatgtg gacgccgggc ccgatcatat tgtcgctcag
     3781 gatcgtggcg ttgtgcttgt cggccgttgc tgtcgtaatg atatcggcac cttcgaccgc
     3841 ctgttccgca gagatcccgt gggcgaagaa ctccagcatg agatccccgc gctggaggat
     3901 catccagccg gcgtcccgga aaacgattcc gaagcccaac ctttcataga aggcggcggt
     3961 ggaatcgaaa tctcgtgatg gcaggttggg cgtcgcttgg tcggtcattt cgaaccccag
     4021 agtcccgctc agaagaactc gtcaagaagg cgatagaagg cgatgcgctg cgaatcggga
     4081 gcggcgatac cgtaaagcac gaggaagcgg tcagcccatt cgccgccaag ctcttcagca
     4141 atatcacggg tagccaacgc tatgtcctga tagcggtccg ccacacccag ccggccacag
     4201 tcgatgaatc cagaaaagcg gccattttcc accatgatat tcggcaagca ggcatcgcca
     4261 tgggtcacga cgagatcatc gccgtcgggc atgcgcgcct tgagcctggc gaacagttcg
     4321 gctggcgcga gcccctgatg ctcttcgtcc agatcatcct gatcgacaag accggcttcc
     4381 atccgagtac gtgctcgctc gatgcgatgt ttcgcttggt ggtcgaatgg gcaggtagcc
     4441 ggatcaagcg tatgcagccg ccgcattgca tcagccatga tggatacttt ctcggcagga
     4501 gcaaggtgag atgacaggag atcctgcccc ggcacttcgc ccaatagcag ccagtccctt
     4561 cccgcttcag tgacaacgtc gagcacagct gcgcaaggaa cgcccgtcgt ggccagccac
     4621 gatagccgcg ctgcctcgtc ctgcagttca ttcagggcac cggacaggtc ggtcttgaca
     4681 aaaagaaccg ggcgcccctg cgctgacagc cggaacacgg cggcatcaga gcagccgatt
     4741 gtctgttgtg cccagtcata gccgaatagc ctctccaccc aagcggccgg agaacctgcg
     4801 tgcaatccat cttgttcaat catgcgaaac gatccagatc cggtgcagat tatttggatt
     4861 gagagtgaat atgagactct aattggatac cgaggggaat ttatggaacg tcagtggagc
     4921 atttttgaca agaaatattt gctagctgat agtgacctta ggcgactttt gaacgcgcaa
     4981 taatggtttc tgacgtatgt gcttagctca ttaaactcca gaaacccgcg gctgagtggc
     5041 tccttcaacg ttgcggttct gtcagttcca aacgtaaaac ggcttgtccc gcgtcatcgg
     5101 cgggggtcat aacgtgactc ccttaattct ccgctcatga tgaattcaga aataaattca
     5161 gcctaattcg gcgttaattc agtacattaa aaacgtccgc aatgtgttat taagttgtct
     5221 aagcgtcaat ttgtttacac cacaatatat cctgccacca gccagccaac agctccccga
     5281 ccggcagctc ggcacaaaat caccactcga tacaggcagc ccatcagtcc gggacggcgt
     5341 cagcgggaga gccgttgtaa ggcggcagac tttgctcatg ttaccgatgc tattcggaag
     5401 aacggcaact aagctgccgg gtttgaaaca cggatgatct cgcggagggt agcatgttga
     5461 ttgtaacgat gacagagcgt tgctgcctgt gatcaattcg ggcacgaacc cagtggacat
     5521 aagcctgttc ggttcgtaag ctgtaatgca agtagcgtat gcgctcacgc aactggtcca
     5581 gaaccttgac cgaacgcagc ggtggtaacg gcgcagtggc ggttttcatg gcttgttatg
     5641 actgtttttt tggggtacag tctatgcctc gggcatccaa gcagcaagcg cgttacgccg
     5701 tgggtcgatg tttgatgtta tggagcagca acgatgttac gcagcagggc agtcgcccta
     5761 aaacaaagtt aaacatcatg ggggaagcgg tgatcgccga agtatcgact caactatcag
     5821 aggtagttgg cgtcatcgag cgccatctcg aaccgacgtt gctggccgta catttgtacg
     5881 gctccgcagt ggatggcggc ctgaagccac acagtgatat tgatttgctg gttacggtga
     5941 ccgtaaggct tgatgaaaca acgcggcgag ctttgatcaa cgaccttttg gaaacttcgg
     6001 cttcccctgg agagagcgag attctccgcg ctgtagaagt caccattgtt gtgcacgacg
     6061 acatcattcc gtggcgttat ccagctaagc gcgaactgca atttggagaa tggcagcgca
     6121 atgacattct tgcaggtatc ttcgagccag ccacgatcga cattgatctg gctatcttgc
     6181 tgacaaaagc aagagaacat agcgttgcct tggtaggtcc agcggcggag gaactctttg
     6241 atccggttcc tgaacaggat ctatttgagg cgctaaatga aaccttaacg ctatggaact
     6301 cgccgcccga ctgggctggc gatgagcgaa atgtagtgct tacgttgtcc cgcatttggt
     6361 acagcgcagt aaccggcaaa atcgcgccga aggatgtcgc tgccgactgg gcaatggagc
     6421 gcctgccggc ccagtatcag cccgtcatac ttgaagctag acaggcttat cttggacaag
     6481 aagaagatcg cttggcctcg cgcgcagatc agttggaaga atttgtccac tacgtgaaag
     6541 gcgagatcac caaggtagtc ggcaaataat gtctagctag aaattcgttc aagccgacgc
     6601 cgcttcgcgg cgcggcttaa ctcaagcgtt agatgcacta agcacataat tgctcacagc
     6661 caaactatca ggtcaagtct gcttttatta tttttaagcg tgcataataa gccctacaca
     6721 aattgggaga tatatcatgc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     6781 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     6841 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     6901 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     6961 tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     7021 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     7081 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     7141 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     7201 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     7261 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     7321 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     7381 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     7441 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     7501 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
     7561 agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt
     7621 gcggtatttc acaccgcata tggtgcactc tcagtacaat ctgctctgat gccgcatagt
     7681 taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc
     7741 gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca
     7801 agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg
     7861 cgcgaggcag ggtgccttga tgtgggcgcc ggcggtcgag tggcgacggc gcggcttgtc
     7921 cgcgccctgg tagattgcct ggccgtaggc cagccatttt tgagcggcca gcggccgcga
     7981 taggccgacg cgaagcggcg gggcgtaggg agcgcagcga ccgaagggta ggcgcttttt
     8041 gcagctcttc ggctgtgcgc tggccagaca gttatgcaca ggccaggcgg gttttaagag
     8101 ttttaataag ttttaaagag ttttaggcgg aaaaatcgcc ttttttctct tttatatcag
     8161 tcacttacat gtgtgaccgg ttcccaatgt acggctttgg gttcccaatg tacgggttcc
     8221 ggttcccaat gtacggcttt gggttcccaa tgtacgtgct atccacagga aagagacctt
     8281 ttcgaccttt ttcccctgct agggcaattt gccctagcat ctgctccgta cattaggaac
     8341 cggcggatgc ttcgccctcg atcaggttgc ggtagcgcat gactaggatc gggccagcct
     8401 gccccgcctc ctccttcaaa tcgtactccg gcaggtcatt tgacccgatc agcttgcgca
     8461 cggtgaaaca gaacttcttg aactctccgg cgctgccact gcgttcgtag atcgtcttga
     8521 acaaccatct ggcttctgcc ttgcctgcgg cgcggcgtgc caggcggtag agaaaacggc
     8581 cgatgccggg atcgatcaaa aagtaatcgg ggtgaaccgt cagcacgtcc gggttcttgc
     8641 cttctgtgat ctcgcggtac atccaatcag ctagctcgat ctcgatgtac tccggccgcc
     8701 cggtttcgct ctttacgatc ttgtagcggc taatcaaggc ttcaccctcg gataccgtca
     8761 ccaggcggcc gttcttggcc ttcttcgtac gctgcatggc aacgtgcgtg gtgtttaacc
     8821 gaatgcaggt ttctaccagg tcgtctttct gctttccgcc atcggctcgc cggcagaact
     8881 tgagtacgtc cgcaacgtgt ggacggaaca cgcggccggg cttgtctccc ttcccttccc
     8941 ggtatcggtt catggattcg gttagatggg aaaccgccat cagtaccagg tcgtaatccc
     9001 acacactggc catgccggcc ggccctgcgg aaacctctac gtgcccgtct ggaagctcgt
     9061 agcggatcac ctcgccagct cgtcggtcac gcttcgacag acggaaaacg gccacgtcca
     9121 tgatgctgcg actatcgcgg gtgcccacgt catagagcat cggaacgaaa aaatctggtt
     9181 gctcgtcgcc cttgggcggc ttcctaatcg acggcgcacc ggctgccggc ggttgccggg
     9241 attctttgcg gattcgatca gcggccgctt gccacgattc accggggcgt gcttctgcct
     9301 cgatgcgttg ccgctgggcg gcctgcgcgg ccttcaactt ctccaccagg tcatcaccca
     9361 gcgccgcgcc gatttgtacc gggccggatg gtttgcgacc gctcacgccg attcctcggg
     9421 cttgggggtt ccagtgccat tgcagggccg gcagacaacc cagccgctta cgcctggcca
     9481 accgcccgtt cctccacaca tggggcattc cacggcgtcg gtgcctggtt gttcttgatt
     9541 ttccatgccg cctcctttag ccgctaaaat tcatctactc atttattcat ttgctcattt
     9601 actctggtag ctgcgcgatg tattcagata gcagctcggt aatggtcttg ccttggcgta
     9661 ccgcgtacat cttcagcttg gtgtgatcct ccgccggcaa ctgaaagttg acccgcttca
     9721 tggctggcgt gtctgccagg ctggccaacg ttgcagcctt gctgctgcgt gcgctcggac
     9781 ggccggcact tagcgtgttt gtgcttttgc tcattttctc tttacctcat taactcaaat
     9841 gagttttgat ttaatttcag cggccagcgc ctggacctcg cgggcagcgt cgccctcggg
     9901 ttctgattca agaacggttg tgccggcggc ggcagtgcct gggtagctca cgcgctgcgt
     9961 gatacgggac tcaagaatgg gcagctcgta cccggccagc gcctcggcaa cctcaccgcc
    10021 gatgcgcgtg cctttgatcg cccgcgacac gacaaaggcc gcttgtagcc ttccatccgt
    10081 gacctcaatg cgctgcttaa ccagctccac caggtcggcg gtggcccata tgtcgtaagg
    10141 gcttggctgc accggaatca gcacgaagtc ggctgccttg atcgcggaca cagccaagtc
    10201 cgccgcctgg ggcgctccgt cgatcactac gaagtcgcgc cggccgatgg ccttcacgtc
    10261 gcggtcaatc gtcgggcggt cgatgccgac aacggttagc ggttgatctt cccgcacggc
    10321 cgcccaatcg cgggcactgc cctggggatc ggaatcgact aacagaacat cggccccggc
    10381 gagttgcagg gcgcgggcta gatgggttgc gatggtcgtc ttgcctgacc cgcctttctg
    10441 gttaagtaca gcgataacct tcatgcgttc cccttgcgta tttgtttatt tactcatcgc
    10501 atcatatacg cagcgaccgc atgacgcaag ctgttttact caaatacaca tcaccttttt
    10561 agacggcggc gctcggtttc ttcagcggcc aagctggccg gccaggccgc cagcttggca
    10621 tcagacaaac cggccaggat ttcatgcagc cgcacggttg agacgtgcgc gggcggctcg
    10681 aacacgtacc cggccgcgat catctccgcc tcgatctctt cggtaatgaa aaacggttcg
    10741 tcctggccgt cctggtgcgg tttcatgctt gttcctcttg gcgttcattc tcggcggccg
    10801 ccagggcgtc ggcctcggtc aatgcgtcct cacggaaggc accgcgccgc ctggcctcgg
    10861 tgggcgtcac ttcctcgctg cgctcaagtg cgcggtacag ggtcgagcga tgcacgccaa
    10921 gcagtgcagc cgcctctttc acggtgcggc cttcctggtc gatcagctcg cgggcgtgcg
    10981 cgatctgtgc cggggtgagg gtagggcggg ggccaaactt cacgcctcgg gccttggcgg
    11041 cctcgcgccc gctccgggtg cggtcgatga ttagggaacg ctcgaactcg gcaatgccgg
    11101 cgaacacggt caacaccatg cggccggccg gcgtggtggt gtcggcccac ggctctgcca
    11161 ggctacgcag gcccgcgccg gcctcctgga tgcgctcggc aatgtccagt aggtcgcggg
    11221 tgctgcgggc caggcggtct agcctggtca ctgtcacaac gtcgccaggg cgtaggtggt
    11281 caagcatcct ggccagctcc gggcggtcgc gcctggtgcc ggtgatcttc tcggaaaaca
    11341 gcttggtgca gccggccgcg tgcagttcgg cccgttggtt ggtcaagtcc tggtcgtcgg
    11401 tgctgacgcg ggcatagccc agcaggccag cggcggcgct cttgttcatg gcgtaatgtc
    11461 tccggttcta gtcgcaagta ttctacttta tgcgactaaa acacgcgaca agaaaacgcc
    11521 aggaaaaggg cagggcggca gcctgtcgcg taacttagga cttgtgcgac atgtcgtttt
    11581 cagaagacgg ctgcactgaa cgtcagaagc cgactgcact atagcagcgg aggggttgga
    11641 tcaaagtact ttgatcccga ggggaaccct gtggttggca tgcacataca aatggacgaa
    11701 cggataaacc t
//
